Effects of Tongqiao Huashuan Decoction on Brain Tissue Ultrastructure of Bolt in Rates with Cerebral Ischemia

Abstract:

Objective: To observe the effect of Miao medicine Tongqiao Huashuan decoction on brain tissue ultrastructure of bolt in rats with focal cerebral ischemia/reperfusion injurys. Method: SD rats were randomly divided into 5 groups (Tongqiao Huashuan decoction high dosage group, middle dose group,low dose group, model group,sham operation group).The middle cerebral artery occlusion (MCAO)was used to establish focal cerebral ischemia-reperfusion model. The corresponding Tongqiao Huashuan decoction (17.50,8.75,4.35 g·kg^-1·d^-1),saline gavage were given for 15 days. The brain tissue superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and ultrastructure of brain tissue were observed. Result: Compared with the sham operation group, MDA in model group was significantly increased, SOD activity was significantly decreased (P<0.05);compared with the model group, Tongqiao Huashuan decoction reduced the MDA content in brain tissues, increased the activity of SOD (P<0.01). Tongqiao Huashuan decoction could reduce the damage of brain tissue after cell apoptosis. Conclusion: High doses of Tongqiao Huashuan decoction could obviously alleviate the degeneration and necrosis of nerve cells and reduce apoptosis,its mechanism may be related to eliminate free radicals.