Effects of Xuanfu Daizhe Decoction on Na+-K+-ATPase and Ca2+-Mg2+- ATPase Activity of Esophageal Mucosa in Reflux Esophagitis Rats

Abstract:

Objective: To observe Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activity of esophageal mucosa in reflux esophagitis(RE) rats, and to investigate the fundamental pathogenesis of RE and the therapeutic mechanism in treating RE with Xuanfu Daizhe decoction. Method: Ninty six male Wistar rats were divided into 6 groups:sham operation group, model group, Xuanfu Daizhe decoction high, middle and low dose group (24,12,6 g·kg^-1)and western medicine (OME 10 mg·kg^-1 plus Mosapride 2 mg·kg^-1) group, 16 rats each group. Esophagus-duodenum anastomosis was used to establish rat RE model. From 3 days after operation, NS was given to the sham operation group rats and model group rats by gavage feeding;Xuanfu Daizhe decoction and western medicine (OME 10 mg·kg^-1 plus mosapride 2 mg·kg^-1) was given to the medicine treated groups, and execute all rats in the 8^th day, then observe mucous gross change of lower esophagus with eyes and optical microscope. At last, detect the expression of enzymatic activity of Na⁺-K⁺-ATP and Ca²⁺-Mg²⁺-ATP by chemical methods. Result: Compared with sham operation group, the mortality of the model group was higher;the pathological changes and score were increased;the Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase of esophageal mucosa were decreased. The mortality of the high and middle dose group of Xuanfu Daizhe decocton treated group was decreased significantly compared with the model group (P<0.05). The rats weight of Xuanfu Daizhe decoction treated group were increased significantly compared with the model group (P<0.05). The pathological changes and score were all reduced in Xuanfu Daizhe decoction high and middle dose group(P<0.01);both Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase of esophageal mucosa were increased in Xuanfu Daizhe decoction high and middle dose group (P<0.01). Conclusion: Xuanfu Daizhe decoction could increase the activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase, and protects the cellular integrity of esophageal mucosa, reducing the esophageal mucosal injury of RE.