Juvenile Male Rats Reproductive Damage by Glycosides of Tripterygium wilfordii and Its Protection Role Bushen Chinese Herbs and Observition Finals Fertility of Male Rats

Abstract:

Objective: The research that the juvenile male rats reproductive damage and injury fertility was impacted by glycosides of Tripterygium wilfordii (GWP) and its protection role Bushen Chinese herbs (Cuscutae Semen flavone, Liuwei Dihuang wan). Method: The first part of reproductive damage and histopathological study group:3-4 week-old SD 48 male rats were randomly divided into 4 groups:control group, GTW, Liuwei Dihuang Wan group,Cuscutae Semen flavone group.After 12 weeks, rats were sacrificed,testicle, epididymis immediately weighed to calculate the viscera index, weighed and placed in fixative save, morphological changes of testicular tissue to be seen;part Ⅱ:cage all rats fed one per cage, an adult female rats were placed in accordance with the ratio of 1:1.Three times in the same cage, each co-cage time is 15 days, the pregnancy rate of mated females observed after pups weaning survival, growth and development of offspring. Result: GTW can make testicular germ cell swelling and degeneration,can make spermatogonia, spermatocytes, sperm cells decreased significantly. Cuscutae Semen flavone, Liuwei Dihuang wan can significantly reduce this damage;after 12 weeks with GWP fed male pups:weight, testicular weight, testicular index, epididymal weight, epididymal index indicators blank group comparison showed no significant difference. Each mated conception rates, weaning survival, growth and development of the pups in each group comparison showed no significant difference. Conclusion: GTW damage to the reproductive system is reversible;pathological damage to reproductive organs can be restored;Bushen Chinese herbs can fight TWP caused reproductive damage;no effect on the fertility of the late cubs growth and development is not impact.