Experimental Study on Intervention of Dachengqi Tang for Liver Apoptosis Induced FADD in Acute Hepatic Failure Rats

Abstract:

Objective: To explore the effect of Dachengqi tang on liver apoptosis induced Fas-associated death domain (FADD) in acute hepatic failure rats. Method: Forty Wistar rats were randomLy divided into 4 groups:normal group(group A), model group(group B),treatment group of Dachengqi tang at low dose(group C), treatment group at high dose(group D).There were 10 rats in every group. The level of tumor necrosis factor-α(TNF-α) was measured,Hepatic FADD,tumor necrosis factor receptor type 1(TNFR1) and Caspase-8 activities were examined,liver cell apoptosis was observed.Liver pathology was observed. Result: Thioacetamide (TAA) could induce acute hepatic failure,with extensive liver apoptosis, the expression of FADD,TNFR1 and Caspase-8 were significantly increased in TAA model group.Compared with group B.the percentage of liver cell apoptosis was decreased in C,D groups, the pathology change of liver was decreased in C,D groups,the level of TNF-α was decreased in C,D groups.The expression of FADD,TNFR1 and Caspase-8 were decreased in C,D groups. Conclusion: Dachengqi tang can prevent and cure liver apoptosis, it may be related to depress rhe liver apoptosis induced FADD.