Preparation of Tanshinone ⅡA-loaded Polylactic Acid-Glycolic Acid Microspheres by SPG Membrane Emulsification Technique

Abstract:

Objective: To prepare tanshinone Ⅱ_A-loaded polylactic acid-glycolic acid(PLGA) microspheres by SPG membrane emulsification technique. Method: Based on single factor tests,with composite score of drug loading,encapsulation efficiency and polydispersity index(PDI) as dependent variable,response surface methodology was adopted to optimize prescription process by taking flow rate of mobile phase,volume ratio of oil-water phase,PLGA and PVA concentration as independent variables. Result: Optimum prescription process was as follows:PLGA concentration 44.29 g·L^-1,circulation speed 825.68 r·min^-1,PVA concentration 2.5 g·L^-1 and volume ratio of oil-water 1 ：7.86;These prepared tanshinone Ⅱ_A-loaded PLGA microspheres had smooth rounded surface and uniform size with average size of 2.338 μm,PDI index of 0.328,drug loading efficiency of 1.20% and encapsulation efficiency of 89.57%,relative errors of these parameters were small by comparing with the predictive values. Conclusion: SPG membrane emulsification technique was simple and effective,it could improve bioavailability of tanshinone Ⅱ_A.