Transport Characteristics of α-Cyperone in Caco-2 Cell Model by LC-MS

Abstract:

Objective: To investigate transport mechanisms of α-cyperone in Caco-2 cell model by LC-MS. Method: Caco-2 cell model was established,effects of volume fraction and time on bidirectional transport process of α-cyperone were investigated.LC-MS was adopted to determine the content of α-cyperone with mobile phase of methanol(A)-water(B)(0-5 min,30%-70%A;5-10 min,70%-100%A;15-20 min,30%A) and a flow rate of 0.25 mL·min^-1,values of apparent permeability coefficient(Papp) under different conditions were calculated. Result: Both transport amounts of AP-to-BL and BL-to-AP increased with increasing of volume fraction and time within 2 h.Papp kept 0.5-1.5 at different volume fractions of α-cyperone. Conclusion: α-Cyperone could be transported through intestinal mucosa via a passive diffusion mechanism.