Investigation of Laboratory Extraction Conditions for Proteins from Sunflower Seed Kernels

Abstract:

Objective: To investigate laboratory extraction conditions of proteins from sunflower seed kernels. Method: Eight different conditions(P1,P2,P3,P4,P5,P6,P7,P8) were adopted to extract proteins from sunflower seed kernels,purity of proteins was determined by Bradford,extraction conditions were analyzed by two-dimensional electrophoresis method. Result: After extracted by eight conditions,content of protein powder in sunflower seed kernels was 57.43-63.59 mg without significant differences;But protein purity of P3,P4,P7 and P8 was significantly higher than the other four conditions;2-D PAGE electrophoresis results showed that P4 and P8 had the most protein spots which were (196±11) and (194±20),respectively;However,protein spots was more clear by comparing with P8.Optimum technology conditions were as follows:extraction temperature 50 ℃,ultrasonic time 20 min,with deionized water as extraction solvent. Conclusion: The amount and kind of most proteins obtained under condition of P4,and it could a reference for proteomics of plant proteins.