Content Determination of Trihydroxybenzoic Acid and Paeonol in Paeonia delavayi from Different Producing Area by HPLC

Abstract:

Objective: To establish determined method of the content of trihydroxybenzoic acid and paeonol in Paeonia delavayi, and compare the content in samples from different areas and samples from different parts. Method: The samples were separated by a ZORBOX SB-C₁₈ (4.6 mm×250 mm, 5 μm) using acetonitrile-0.1% methane acid with water gradient system as mobile phase. The flow rate was 1.0 mL·min^-1, and the detection wavelength for fingerprinting was 275 nm with 30 ℃ for column temperature. Result: There were well linear relationships for trihydroxybenzoic acid and paeonol in range of 0.204 0-2.040 μg and 0.204 0-2.040 μg. And average recovery rates for them (n=6) were 101.81% and 97.80%. Conclusion: The method could be used for quality control to medicinal materials of P. delavayi. Contents of trihydroxybenzoic acid and paeonol were significantly different for medicinal materials collected from different areas.