Rapid Determination of Six Lignans in Schisandra Chinensis by Micellar Electrokinetic Capillary Chromatography

Abstract:

Objective: A micellar electrokinetic capillary chromatography(MEKC) was developed for the rapid determination of six lignans in Schisandra chinensis. Method: The key factors for separation and determination were studied and the best analysis conditions were obtained, in the background electrolyte of 10 mmol·L^-1 phosphate(35% acetonitrile, 37.5 mmol·L^-1 SDS, pH 8.0) at the separation voltage of 28.0 kV, the Schisandra extract could be detected within 6 min. Result: The lignans showed good linear response in the range of 25.5-1 020,25.75-1 030,12.0-480,25.5-1 020,24.25-970 and 19.75-790 mg·L^-1, the limit of detection was 0.5,0.8,1.0,1.0,1.0 and 1.5 mg·L^-1. Conclusion: Compared with high-performance liquid chromatography(HPLC), the MEKC analyses offers high separation efficiencies and rapid analyses.

Key words:Micellar electrokinetic capillary chromatography(MEKC);Schisandra chinensis;lignans