Simultaneous Determination of Paeoniflorin,Baicalin and Berberine in the Mixed Decoction of Huanglian Ejiaotang by HPLC

Abstract:

Objective: A simple and rapid HPLC method was established for simultaneously determining of paeoniflorin,baicalin and berberine in the mixed decoction of Huanglian Ejiaotang. Method: Agilent TC-C₁₈(4.6 mm×250 mm)chromatographic column,acetonitrile-0.1% phosphoric acid solution gradient elution were applied. Analyses were performed at 35 ℃ with a flowrate of 1.0 mL·min^-1 and UV detection at 240 nm, Result: The content of paeoniflorin,baicalin and berberine were 1.64%,7.51%,3.30% respectively. Conclusion: The developed method can be applied to the intrinsic quality control of Huanglian Ejiaotang.