HPLC Fingerprint of Monochasma sheareri

Abstract:

Objective: To establish the chromatographic fingerprint for Monochasma sheareri. by RP-HPLC. Method: The HPLC fingerprint of M. sheareri were determined on an Agilent HC C₁₈ column (4.6 mm×250 mm,5 μm) eluted with the mobile phase consisted of acetonitrile and 0.4% phosphoric acid in gradient mode, flow rate 0.8 mL·min^-1,column temperature 35 ℃and the detection wavelength was set at 254 nm.The chromatograms of different batches of M. sheareri. were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A). Result: HPLC Fingerprint of M. sheareri. was established with luteolin as the reference compound. 13 common peaks were selected as the fingerprint peaks of M. sheareri. Conclusion: The established HPLC fingerprint of M. sheareri. has desirable precision,reproducibility,and can be applied to routine quality control of M. sheareri.