Chemical Components, Cytotoxic and Antimicrobial Activity of Essential Oil of Leaves of Poncirus trifoliata

Abstract:

Objective: To study the chemical constituents and bioactivity of essential oil from Poncirus trifoliata. Method: The essential oil was extracted by hydrodistillation and separated by capillary GC. The chemical constituents were determined by normalization and were identified by MS. Result: 65 chromatographic peaks were detected and 51 compounds were identified, which were 91.25% of the total essential oil. The cytotoxicity of the essential oil against human cancer cell lines BEL-7402, K-562, SGC-7901 and SPCA-1 was investigated in vitro. Conclusion: The major components of the essential oil were linalool oxide (11.93%), (-)-globulol (10.18%) and Ledol (8.92%). The oil showed moderate activity against K-562 and BEL-7402 human tumor cell lines with IC₅₀ values less than 40 μg/ml. The oil was also found to possess antimicrobial activity against Bacillus subtilis.