Metabonomic Study of Intervention Effects of Qinghuoling on Stagnant Heat Syndrome in Rats

Abstract:

Objective: To investigate the metabolic profile of stagnant heat syndrome in rats and the intervention effects of Qinghuoling. Method: There were control, model and Qinghuoling groups.Model and Qinghuoling groups were treated with special feed and ip atropine (0.25 mg·kg^-1),twice a day, continues modeling for 7 days, from the 4 th day of modeling, ig Qinghuoling(2.64 g·kg^-1),once a day.Proton nuclear magnetic resonance(¹H-NMR) technique was used to analyze the rat serum and urine metabolic profiles. Multivariate data analysis methods such as orthogonal partial least squares discriminant analysis (OPLS-DA) and principal components analysis (PCA), and univariate statistical analysis methods were used to analyze the metabonome difference between the control and model group samples, to identify the corresponding metabolic biomarkers and to study the intervention effect of Qinghuoling. The mechanism of action of Qinghuoling was illustrated through the selected biomarkers. Result: Compared with the control group, rectal temperature, water intake, and food intake of model group has a significant change The metabolic phenotype of rats in model group greatly changed, Lipid metabolism, glucose uptake and metabolism, intestinal flora have been affected in different degrees and Qinghuoling had efficacious intervention. Six and sevenpotential biomarkers were selected in rat serum and urine, respectively. Conclusion: Qinghuoling can effectively improve the disturbance of lipids and glucose metabolismand modulate the gut microflora environment in rats.