Effects of Zhali Nusi Fang on Nerve Growth Factor Expression in Rat Brain after Cerebral Ischemia Reperfusion

Abstract:

Objective: To observe the effects of Zhali Nusi fang(ZLNSF) on nerve growth factor(NGF) expression in the rat brain after cerebral ischemia reperfusion. Method: Rat focal cerebral ischemia reperfusion model was established by middle cerebral artery occlusion (MCAO) with nylon thread.The animal models were randomized into sham-operation,model, nimodipine(NMDP) and ZLNSF 4 groups to receive treatment with normal saline, NMDP and ZLNSF 30 min after the reperfusion. To do neurological score before take material.To take material at 1,3,7,14 days.Immunohistochemistry was employed to detect the expressions of nerve growth factor at rat brain cortex. Result: The neurological scores of model group rats are reduce, the number of nerve cells are cut down with unequal size and pathological damage significant. compared with model groups, the neurological scores of ZLNSF and NMDP groups are raise, the number of nerve cells are raise, pathological damage brain tissue are reduce significantly and NGF expression are enhance (P<0.05). Compared with NMDP groups, the level of NGF expression was significantly higher in ZLNSF 14 days group (P<0.05). Conclusion: ZLNSF show protective effects against global ischemia-reperfusion injury,ZLNSF 14 days group has the highist cerebral protective effects, and its cerebral protection mechanism may be related to regulate NGF expression in rat brain.