Effect of New Formulation of K-CoxB-JN Compound on TNF-α and IL-6 in Mice with Coxsackie B3 Viral Myocarditis

Abstract:

Objective: To investigate the effect and its mechanisms of the new formulation of K-CoxB-JN compound on tumor necrosis factor-α(TNF-α) and interleukine(IL)-6 in mice with coxsackie B3 viral myocarditis. Method: The mice were injected with CVB3 to establish the experimental model of viral myocarditis,then randomly divided into 5 groups:The new formulation of K-CoxB-JN compound low,middle,high dose group (0.2,0.4,0.8 g·kg^-1),ribavirin group(0.075 g·kg^-1), the model group.and set up the normal group,two houres later mice were gavaged for 14 days. The general state of the mice were observed, deaths were recorded and calculated the survival rate of mice;the content of TNF-α, IL-6 were tested. Took heart and calculated the cardiac index, HE staining,calculated the pathological integral. Result: Compared with the normal group,the model group mice cardiac index,TNF-α and IL-6 level, the pathological integral were increased(P<0.05), the survival rate were decreased(P<0.05),Compared with the model group, the new formulation of K-CoxB-JN compound group mice cardiac index,TNF-α and IL-6 level, the pathological integral were decreased(P<0.05), the survival rate were increased(P<0.05). Conclusion: Regulation of TNF-α, IL-6 level may be one of the mechanisms of the new formulation of K-CoxB-JN compound in the treatment of viral myocarditis.