Optimization of Ultrasound Extraction Technology for Total Saponins from Asparagus officinalis by Box- Behnken Response Surface Methodology

Abstract:

Objective: To optimize ultrasound extraction technology of total saponins from Asparagus officinalis. Method: With the content of total saponins as index, effects of ethanol concentration, solid-liquid ratio, ultrasonic time and temperature on extraction technology were investigated by single factor tests and Box-Behnken response surface methodology. Result: Optimum ultrasound extraction technology was as following:ultrasonic extracted 54 min with 15 times the amount of 74% ethanol at 50 ℃;Under these conditions, the average mass fraction of total saponins was 13.059%, which was wery close to the predicted value of 13.185%. Conclusion: This optimized technology was stable and feasible to provide a basis for development and application of total saponins in A. officinalis.