Optimization of Semi-bionic Extraction Technology for Compound Tianma Gouteng Orally Disintegrating Tablets

Abstract:

Objective: To optimize semi-bionic extraction technology of compound Tianma Gouteng orally disintegrating tablets. Method: With composite score of gastrodin, rhomotoxine, paeoniflorin and ferulic acid extraction amounts as index, L₉(3⁴) orthogonal test was adopted to investigate effects of the amount of water, extracting time and temperature on semi-bionic extraction technology.HPLC was used to determine contents of gastrodin, paeoniflorin and ferulic acid, mobile phase were acetonitrile-0.05% phosphoric acid(3:97), acetonitrile-0.1% phosphoric acid(14:86) and acetonitrile-0.1% phosphoric acid(17:83), detection wavelength were 220, 230 and 316 nm, respectively;The content of rhomotoxine was determined by UV. Result: Optimum extraction technology was as follows:extracted 2 h with 12 times the amount of water at 60 ℃;Total extraction amounts of gastrodin, rhomotoxine, paeoniflorin and ferulic acid in five times the amount of prescriptions medicines were 0.479, 1.276, 4.802 and 0.061 g, respectively. Conclusion: This optimized extraction process was stable and feasible, which was suitable for industrial production of compound Tianma Gouteng orally disintegrating tablets.