Optimization of Purification Process for Total Organic Acids from Roots of Aralia cordata

Abstract:

Objective: To optimize purification process of total organic acids from roots of Aralia cordata. Method: The content of total organic acids was determined by acid-base titration.With purity and transferring rate of total organic acids as indexes, combining with organic solvent extraction and acid-base method, effects of extraction solvents, reagent for alkalization and its concentration, alkalinizing times and pH of acid settling on purification process of total organic acids were investigated by single factor tests. Result: Optimum purification technology was selected as follows:extracted four times with two-fold volume of petroleum ether, 1.0% KOH as alkalization reagent, three times for alkalization, pH 1 of acid settling by hydrochloric acid, extracted three times with petroleum ether after acid settling;Under these purification conditions, purity of total organic acids increased from 14.71% in extract to 65.08% with transferring rate of 76.17%. Conclusion: This purification process was stable and feasible, which could provide experimental evidence for comprehensive utilization of A. cordata resources.