Optimization of Extraction Process of Fritillariae Cirrhosae Bulbus Tissue Culture Materials

Abstract:

Objective: To optimize extraction process of Fritillariae Cirrhosae Bulbus tissue culture materials. Method: UV was adopted to determine the content of total alkaloids with detection wavelength at 415 nm.With the content of total alkaloids as index, extraction method was selected by single factor test, orthogonal design was used to investigate effects of the concentration and amount of ethanol, extraction time and frequance on extracting process. Result: Reflux method was selected to extract Fritillariae Cirrhosae Bulbus tissue culture materials, optimal extracting process was as follows:extracted twice with 6 times the amount of 85% ethanol, 1.5 hours for each time;Extraction amount of total alkaloids was 0.197 g·g^-1. Conclusion: This technology was stable and feasible, which could be as preparation process of Fritillariae Cirrhosae Bulbus tissue culture materials extract.