Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology

Abstract:

Objective: To optimize purification process of total flavonoids in Aurantii Fructus Immaturus by macroporous resin. Method: Independent variables were adsorption time, ethanol concentration and eluent volume, while dependent variables was adsorption-elution rate of total flavonoids, central composite design-response surface methodology was adopted to optimize purification technology on the basis of single factor tests. Result: AB-8 macroporous resin was selected, optimum purification process were as follows:added 0.6 BV of sample solution with concentration of 3.5 g·L^-1 and pH 6.0, sample rate 1 BV·h^-1, adsorption time 77 min, water consumption 1 BV, elution rate of 2 BV·h^-1, eluted with 2.4 BV of 88% ethanol.Adsorption-elution rate of total flavonoids was up to 93.0%, which had a small deviation with the predicted value of 92.4%. Conclusion: Optimized purification technology was stable and feasible with high predictability.