Optimization of Extraction Process of Chrysanthemi Flos by Box-Behnken Response Surface Methodology

Abstract:

Objective: To optimize extraction process of Chrysanthemi Flos. Method: Based on single factor test, taking extracting time, ethanol concentration and amount as independent variables, overall desirability(OD) of yield of luteolin, apigenin and chlorogenic acid as dependent variable, Box-Behnken response surface methodology was applied to optimize extraction conditions. Result: Optional extraction process was as follows:extracted 110 min with 12 times the amount of 80% ethanol;Average yields of luteolin, apigenin and chlorogenic acid were 1.64%, 0.94% and 3.95%, respectively, deviation between the measured value(0.979 6) and the predicted value(0.981 6) of OD was 0.204%. Conclusion: This optimized extaction technology was simple and reliable with high predictability.