HPLC Determination of Three Alkaloids in Aconitum Brachypodum

Abstract:

Objective: To establish a method for the determination of aconitine, mesaconitine and bullatine A in Aconitine brachypdum. Method: A C₁₈ column(4.6 mm×250 mm, 5 μm) was used at 30 ℃ with methanol-0.01% triethylamine (73:27) as the mobile phase at the flow rate of 1.0 mL·min^-1 and UV detection wavelength was set at 240 nm. Result: The average recovery of three alkaloids was 98.88%(RSD 0.86%), 98.68%(RSD 0.48%), and 98.94%(RSD 0.65%) respectively. Conclusion: The method is simple, accurate and reproducible, which can be used in the content determination of three alkaloids in A. brachypdum and provide a more reasonable reference to control the quality of A. brachypdum.