Content Determination of Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Shenmai Injection by UPLC

Abstract:

Objective: To establish a method to determine the content of ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ in Shenmai injection by ultra performance liquid chromatography(UPLC). Method: ACQUITY^® UPLC HSS T3 column (2.1 mm×50 mm, 1.8 μm)was used with mobile phase consisted of acetonitrile (A)-0.05% phosphoric acid solution (B) by gradient elution (0-5 min, 19%A;5-12 min, 19%-28%A;12-18 min, 28%-40%A) at a flow rate of 0.3 mL·min^-1;the wavelength of detector was 203 nm. Result: Ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁had good linearity within the range of 0.020 96-0.209 6, 0.017 64-0.176 4, 0.023 88-0.238 8 g·L^-1 respectively. The average recoveries(n=6)were 100.12%, 100.08%, 99.51% and RSDs were 1.60%, 2.03%, 1.15% respectively. Conclusion: UPLC method may greatly improve the separation efficiency and analysis speed in the case of ginsenoside Rg₁, ginsenoside Re and ginsenoside Rb₁ in Shenmai injection while reducing the solvent consumption. As an alternative of conventional HPLC, UPLC is more convenient, rapid, accurate and excellent repeatability.