Study on the Multi-wavelength HPLC Fingerprinting of Scrophulariae Radix

Abstract:

Objective: Using the multi-wavelength high performance liquid chromatography(HPLC) for testing 10 Scrophulariae Radix samples from Zhejiang Panan, Hubei Enshi, ect. To provide a new method for scientific evaluation and effective control of its quality, and establish the multi-wavelength HPLC fingerprinting of Scrophulariae Radix from different places. Method: Chromatographic conditions:Thermo C₁₈ column, gradient elution was applied with acetonitrile and 0.1% methane acid as the mobile-phase. Ultraviolet (UV) was used combined with multiple wavelength to detect the chromatographic peaks that cannot be displayed in the same channel. The wavelength timetable was as follows, from 0 to 5 min the wavelength was 230 nm;from 5 to 13 min was 210 nm;from 13 to 23 min was 320 nm;from 23 to 50 min was 278 nm. The flow rate was 1 mL·min^-1 and the column temperature was maintained at 30 ℃. Result: A HPLC fingerprinting pattern was set up based on multi-wavelength combined with UV, which demarcated 25 commom peaks and confirmed 6 peaks. The index similarty of those Scrophulariae Radix fingerprinting which analysised through similarity evaluation system for chromatographic fingerprint of TCM (Version 2004 A) were all greater than 0.90. Conclusion: The approach of multi-wavelength HPLC will help to differentiate and evaluate the quality of Scrophulariae Radix. The method is highly sensitive, characteristic peaks of large quantity, chromatographic large amount of information that will help evaluate the quality of Scrophulariaceae Radix.