Separation and Purification of Antibacterial Polysaccharides from Paederia scandens in vivo

Abstract:

Objective: The polysaccharide was separated and purified from Paederia scandens for antibacterial polysaccharide in vivo. Method: The crude polysaccharides were separated and purified by column chromatography, and then the homogeneous polysaccharides were identified by gel column chromatography and polyacrylamide disc gel electrophoresis. Their structure was analyzed by GC and IR. The activities of polysaccharides were studied by antibacterial model in vivo. Result: Two kinds of homogeneous polysaccharides, PSP2a and PSP2b, were obtained from P. scandens. PSP2a were consisted of glucose, galactose, mannose, xylose and arabinose in the molar ratio of 4.92:3.23:1.00:1.73:3.50.PSP2b were consisted of arabinose, galactose and glucose in the molar ratio of 1.00:0.73:1.80.PSP2a and PSP2b mainly contained furanose. The antibacterial model in vivo indicated that PSP, PSP2 and PSP2a could protect mice infected with the resistant strain of P. scandens, and protection of PSP2a was best. Conclusion: The antibacterial component among polysaccharides from P. scandens was mainly PSP2a.