Comparison of Matrine and Oxymatrine in Radix Sophorae Subprostrata of Different Growing Years

Abstract:

Objective: To study the content of matrine and oxymatrine in Radix Sophorae Subprostrata of different growing years. Method: HPLC was adopted, the separation was performed on a welch materials XB-C₁₈ column, acetonitrile-0.08% triethylamine(12:88) was used as the mobile phase, the flow rate was 0.8 mL·min^-1, detection wavelength was set at 220 nm with temperature at 25 ℃. Result: The diversity of the content of matrine and oxymatrine in Radix Sophorae Subprostrata of different growing years was great. The ingredients in 1-2 years of Radix Sophorae Subprostrata were in the lower levels, the ingredients in 3 years increased significantly and the ingredients in over 3 years had stable change. Conclusion: The changing rules of matrine and oxymatrine provide scientific basis for the collecting years.