Determination of 4-Cholesten-3-one and Cholesterol in Oviductus Ranae by HPLC

Abstract:

Objective: To determine the content of 4-cholesten-3-one and cholesterol in Oviductus Ranae by HPLC at the same time. Method: Agilent TC-C₁₈ column (4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of methanol-water (91.5:8.5). The column temperature was maintained at 35 ℃. The volumetric flow rate was set at 1.0 mL·min^-1. The UV detector was set at 215 nm. The sample size was 10 μL. Result: 4-cholesten-3-one was linear in the content range of 0.011 65-0.116 5 μg;the average recovery was 100.0%, RSD 1.83%. Cholesterol was linear in the content range of 2.282-22.82 μg;the average recovery was 99.3%, RSD 1.19%. Conclusion: The method, which is fast, accurate and stable, can simultaneously determine the content of 4-cholesten-3-one and cholesterol in Ranae Oviductus.