Study on Qualtaive Method of Salidroside of Artifical Cultivation Ultivation Rhodiola Rosea by HPLC-MS

Abstract:

Objective: To determine the petals, stems, roots planted content Rhodiola different medicinal parts。Method: Each 1 g sample is dissolved after 60% ethanol, ultrasonic cleaner cleaning 50 min, filtered, rotary evaporated, add 2 mL of methanol, microporous filter membrane (0.45 μm) filter using CAPCELL PAK C₁₈ column ((2.0 mm× 150 mm, i.d., 3 μm ), column temperature 30 ℃, wavelength 278 nm, sample temperature 10 ℃, injection volume of 10 μL. The mobile phase was methanol-1% acetic acid solution 45:55, flow rate of 1 mL·min^-1 for quantitative analysis. Result: The effective parts of salidroside content in the order of the petals (35.00 mg), root (18.89 mg), stems (1.59 mg). Conclusion: A detection of salidroside content in Rhodiola rosea was established by high performance liquid chromatography. It can be applied to the analysis on the salidroside in the different medicinal materials.