A Quantitative Method for Simultaneous Assay of Seven Flavonoids Ingredients with One Marker in Ginkgo biloba Extract by QAMS

Abstract:

Objective: To establish a quantitative method of multi-components by single marker(QAMS) for determining of seven flavonoids for the improvement of the quality control of Ginkgo biloba extract, as well as ensure the accuracy and feasibility of the method apply to G. biloba extract. Method: With the typical composition quercetin as the indexes, the relative correction factors(RCF) between the quercetin and the other six flavonoids were established to detect the quantitation of quercetin and calculate the quantitation of the other six constituents. The external standard method was used for quantitating the seven constituents, and contrasted calculated value with estimated value by Pearson correlation coefficient. Result: Calculated value and estimated value did not make significant difference on QAMS and external standard method. And it showed that the RCF was authentic. Conclusion: In the case of lacking reference substance, the quantitation of quercetin could be detected. It is feasible for taking advantage of the RCF to quantitate seven flavonoids.