Determination of Imperatorin and Isoimperatorin in Fengshiding Capsule by HPLC

Abstract:

Objective: To establish a HPLC method for determination of the content of imperatorin and isoimperatorin in Fengshiding capsule. Method: Hypersil ODS2 column (4.6 mm×150 mm, 5 μm) was used. The methanol-water (62:38) was used as mobile phase. The flow rate was 1.0 mL·min^-1, the detection wavelength was set at 254 nm. The column temperature was set at room temperature. The injection volume was 20 μL. Result: The linear range of imperatorin was 1.064-31.92 mg·L^-1 (r=0.999 7). The linear range of isoimperatorin was 1.090-32.70 mg·L^-1 (r=0.999 3). The average recovery of imperatorin was 100.2% (n=9), RSD 1.47%. The average recovery of isoimperatorin was 100.0% (n=9), RSD 1.64%. Conclusion: The method is simple, sensitive, and accurate for the quality control of Fengshiding capsule.

Key words:HPLC;Fengshiding capsule;imperatorin and isoimperatorin