Optimial Conditions for Fermentation of Ganoderma lucidum and Analysis of the Active Components of the New Fungal Substance

Abstract:

Objective: Corn silk and cob were fermented by Ganoderma lucidum with two-way pattern solid-state fermentation technique and produced new substance, the active compositions were investigated. Method: The optimal ratio of medium was selected according to the changes of polysaccharides and triterpene after fermented. Qualitative and quantitative analysis were established using UV, TLC and HPLC. Result: Ganoderma lucidum was fermented in the media of corn silk and corn cob at 1:0, 1:1, 1:2, 1:3, 1:4, 1:5, 0:1 ratio respectively. The content of polysaccharides increased and the content of triterpene decreased in turn. The result of TLC demonstrated that the color of TLC spot changed from red into blue-green fluorescence after fermentation, and the spot became brighter with increasing proportion of corn silk. The result of HPLC exhibited that some peaks of G. lucidum became weak or disappeared, and some new peaks were found. Conclusion: The new medical fungal substance grew best and contained the highest content of active components with the medium containing corn silk-corn cob (1:5), and the composition of the fungal substance changed significantly after fermentation.