Determination of Plasma Protein Binding Rate of Pulsatilla Saponin D in Several Species

Abstract:

Objective: To investigate different plasma protein binding rates of pulsatilla saponin D. Method: HPLC and equilibrium dialysis method were adopted to determine plasma protein binding rates of pulsatilla saponin D in bovine serum, human and rat plasma, COSMOSIL 5C₁₈-MS-Ⅱ column(4.6 mm×250 mm, 5 μm) was employed with mobile phase of methanol-water-formic acid(80:20:0.02) and detection wavelength at 203 nm. Result: Plasma protein binding rates of pulsatilla saponin D at three various concentrations (0.06, 0.11, 0.25 g·L^-1) were (78.45±0.89)%, (77.61±1.14)%, (77.16±0.29)% in bovine serum albumin, they were (68.91±0.54)%, (67.68±0.87)%, (67.88±0.71)% in human plasma and (78.15±0.76)%, (78.61±0.97)%, (78.24±0.93)% in rat plasma, respectively. Conclusion: This method for determining plasma protein binding rates of pulsatilla saponin D was simple, stable and reliable.Pulsatilla saponin D was a drug with moderate plasma protein binding rate, which did not have concentration-dependent manner.