Effects of Sophoridine, Matrine and Aloperine on the Contents of Cytokine IL-6 and TNF-α in LPS-induced IEC-6 Cells Inflammatory Model

Abstract:

Objective: To observe the protective effects of sophoridine, matrine and aloperine on lipopolysaccharide (LPS)-induced IEC-6 cells inflammatory model. Method: IEC-6 cells were seeded into 12-well plates at the density of 2×10⁵/well and treated with LPS for 12 h. Then, the inflammatory model was constructed by detecting the content of interleukin(IL)-6 and tumor necrosis factor-α(TNF-α). MTT assay was used to determine the activities of aloperine, sophoridine and matrine on LPS-injured IEC-6 cells. Enzyme-linked immunosorbant assay(ELISA) was used to detect the content of IL-6 and TNF-α in cell supernatant and Western blotting analysis was employed to detect the protein expression levels of IL-6 and TNF-α. Result: Compared with the control group, levels of IL-6 and TNF-α of inflammatory model group were significantly upregulated. Aloperine, sophoridine and matrine could significantly reduce the cytotoxicities of IEC-6 cells induced by LPS. The cell viabilities were increased from 80.47% to 91.96%, 92.40% and 97.23% after sophoridine, matrine and aloperine treatment at the concentration of 100 μmol·L^-1, respectively. Moreover, ELISA and Western blotting analysis results demonstrated that these compounds could attenuate the content and expression of IL-6 and TNF-α. Conclusion: Aloperine, sophoridine and matrine exhibit anti-inflammatory effects though decreasing the activities of IL-6 and TNF-α. Among them, aloperine shows the best activities.