Effect and Mechanism of Ginsenosides Rb1 on Insulin Signaling Pathway in Type 2 Diabetes Mice with Memory Impairment

Abstract:

Objective: To study the effect and mechanism of ginsenosides Rb₁ on memory impairment of type 2 diabetes. Method: C57BL mice were as normal control group and Kkay mice with high blood sugar as model l group. The model mice were divided into positive group and composite group with high, middle and low dose. The positive group was treated with Rosiglitazone maleate tablets(0.003 g·kg^-1)ig, and composite group was given the same Rosiglitazone tablets and ginsenosides Rb₁ (0.06, 0.03, 0.015 g·kg^-1) ig. After Morris water mazeimmunohistochemistry was used to assay 1-phosphatidy linosital 3-kinase(PI-3K)/Akt, and Western blotting was used to detect glycogen synthase kinase-3β GSK-3β, and p-Tau and insulin receptor(INSR) level. Result: INSR levels were almost the same in every group.Compared with normal control group, model group had more active PI-3K/Akt and lower GSK-3β and p-Tau level. Howere, compared with control group, there was no significant differences in positive group, while PI-3K/Akt was more active, and GSK-3β and p-Tau was contrary in composite group. Conclusion: Ginsenosides Rb₁ can improve the memory of type 2 diabetes mice, the possible mechanism may be related to improving insulin signaling pathway.