Expression of CD11b mRNA in Central Nervous System and Effect of Yishen Daluo Decoction in Experimental Autoimmune Encephalomyelitis

Abstract:

Objective: To elucidate the CD11b mRNA expression of brain and spinal cord tissue during the different phrase in experimental autoimmune encephalomyelitis (EAE) mice, and to explore the mechanism of Yishen Daluo decoction (YSDLD) on EAE. Method: C57BL/6 mice were immunized with the MOG_35-55. Mice were divided randomly into normal-group, adjuvant group, EAE-group, prednisone-group, YSDLD-goup. RT-PCR was used to quantify the gene expression levels of immune/inflammatory cell (the monocyte marker CD11b), accumulated in brain and spinal cord tissue of mice at different time points of EAE including pre-onset (on day 7th), onset(on day 14th), peak (on day 24th)and chronic phrase (on day 40th) after immunization. Result: Compared with model group, CD11b mRNA levels in the cerebrum were significantly decreased 40 days after immunization in hormone-treated group and YSDLD-treated group. CD11b mRNA levels in the spinal cord were significantly decreased 14 days after immunization in hormone-treated group. Conclusion: The monocyte is one of potential mediators of EAE. The therapeutic mechanism of YSDLD is to mediate the expression of CD11b on the surface of monocyte.