To Compare Effect of Buzhong Yiqi Decoction Serum with siRNA on MRP Expression of A549/DDP Cells

Abstract:

Objective: To compare the effect of Buzhong Yiqi decoction serum with small interfering RNA (siRNA)on the multidrug resistance-associated protein(MRP)expression of A549/DDP lung drug resistance cells. Method: Buzhong Yiqi decoction serum was prepared. 18 rats were divided into the blank group (normal saline, 10 mL·kg^-1), high (11.34 g·kg^-1) and low (2.84 g·kg^-1) dose groups of Buzhong Yiqi decoction. At the end of the 3th day serum was obtained. siRNA was used to silence MRP gene into A549/DDP cells. Reverse transcription-polymerase chain reaction(RT-PCR) and Western blot analyses were performed for MRP mRNA, and MRP expression in A549/DDP cells. Cells were randomized into control groups;Buzhong Yiqi decoction lower does groups;Buzhong Yiqi decoction higher does groups, and MRP siRNA groups. Result: This study showed that three treatment groups could significantly decreased the MRP mRNA expression, and the expression of MRP at the level of protein(P<0.05). Conclusion: These results suggest the Buzhong Yiqi decoction serum could decrease the expression of MRP in A549/DDP cells, and has similar effect with siRNA-mediated specific gene silencing in A549/DDP cells.