Abstract:Objective: To investigate the effect of Pabing 2 formula on the expression of nuclear factor E2-related factor 2(Nrf2)and the hemeoxygenasel(HO-1) in substantia nigra tissue of Parkinson disease(PD) rats model induced by 6-hydroxydopamine(6-OHDA). Method: The rats were stereotaxically injected with 6-OHDA solution into the left striatum in two-site.Rats showed consistently right whirling and the number of rotation was more than 210 r·30 min-1 induced by APO, then the rat was judged as PD model.Twenty-four modeling rats successfully were randomly divided into four groups:model group, Pabing 2 formula(8.0, 16.0, 32.0 g·kg-1), at the same time, the normal group and sham group were established and given distilled water at the same volume, all with a course of 4 weeks. The nuclear Nrf2 protein and HO-1 protein levels of the substantia nigra tissue were detected by Western blot, and the mRNA levels of Nrf2 and HO-1 were determined with RT-PCR. Result: Compared with sham group, the nuclear Nrf2 protein and HO-1protein in substantia nigra tissue were significant increased(P<0.01), and the mRNA levels of Nrf2 and HO-1 were increased in model group(P<0.01). Compared with model group, the nuclear Nrf2 protein and HO-1 protein in substantia nigra tissue were significantly increased (P<0.01), and the mRNA levels of both Nrf2 and HO-1were also upregulated in Pabing 2 formula low dose group, media dose group and high dose group(P<0.01), Pabing 2 formula high dose group were significantly differences compared with the low dose group and media dose group (P<0.01). Conclusion: After 6-OHDA injection, the Nrf2/HO-1 signal transduction pathway is activated.Pabing 2 formula can enhance antioxidation ability.The underlying mechanism might be associated with activating Nrf2/HO-1 signal transduction pathway, promoting the nuclear Nrf2 protein and Nrf2 mRNA, then advancing the expression of antioxidant gene in the downstream such as HO-1 protein and HO-1 mRNA.The effects were more obvious in Pabing 2 formula high dose group.
