Effect of Ampelopsin on HSC-T6 Cell Proliferation and Content of Collage and Cytokine

Abstract:

Objective: To study the effect of ampelopsin on the HSC-T6 cell proliferation rate and the content of collage and cytokine Method: Effect of ampelopsin on the cell (HSC-T6) proliferation rate. High glucose DMEM medium containing 10% fetal bovine serum was used to prepare HSC-T6 cells 3×10⁴ cells/mL suspension 100 μL/well, which was seeded in 96-well culture plates. The control group, colchicine 1.0 mg·L^-1 group and 100.0, 75.0, 50.0, 25.0, 12.5 mg·L^-1, 5 different concentrations of ampelopsin groups were designed. Cells were cultured at 37 ℃ 5%CO₂ for 48 h. The cell proliferation inhibition rate was measured by MTT。Effect of ampelopsin on the content of collage(Ⅰ, Ⅲ, Ⅳ) and cytokine (TGF-β₁, PDGF) was dected by ELISA. Result: Ampelopsin at 100.0, 75.0 mg·L^-1 might effectively inhibit proliferation of HSC-T6 cells, and the non-toxic drug concentration was 50.0 mg·L^-1, the contents of collage Ⅰ, Ⅲ, Ⅳ and TGF-β₁, PDGF in HSC-T6 cells were significantly lower than control group at 50.0 mg·L^-1 of ampelopsin (P<0.05), the contents of collage Ⅲ in HSC-T6 cells was significantly lower than control group by ampelopsin at 25.0 mg·L^-1(P<0.05). Conclusion: Ampelopsin could inhibit the proliferation of HSC-T6 and reduce the content of collage Ⅰ, Ⅲ, Ⅳ and TGF-β₁, PDGF.