Abstract:Objective: To assess the anti-diarrhea, astringing intestine, analyesia anti-nociception and immunity pharmacodynamics of Cangpo oral liquid. Method: Fifty mice were randomly divided into five groups:control group(normal saline), positive control group and low, middle, high dosage groups of Cangpo oral liquid (5, 10, 20 g·kg-1). The effect on the diarrhea induced by Senna leaf was evaluated by the amount of mouse wet dejecta. The effect on the propulsive motility of small intestine was evaluated by push distance of the charcoal powder in intestine. Xylene-induced mouse ear edema was conducted to assess the anti-inflammatory effect of the liquid. Hot plate test were conducted to assess the anti-nociception effect of the liquid. Immunity function was investigated by the phagocytes' phagocytosis of chicken RBC on animal model of spleen deficiency caused by Rhubarb. Result: Compared with the control group, Cangpo oral liquid reduced the amount of mouse wet dejecta (P<0.01, P<0.05), the most significant effect happened in 2 hours after administration. Cangpo oral liquid debased push distance of the charcoal powder in intestine, the high-dose group played the strongest role in three groups (P<0.01). Cangpo oral liquid could inhibit the mice auricle swelling by xylene (P<0.05, P<0.01), and acetate prednisone showed slightly stronger effect than the liquid. Cangpo oral liquid also delayed the latencies of licking paws and prolonged pain threshold in hot plate pain test in mice (P<0.05, P<0.01). In addition, the liquid improved the phagocytic index of peritoneal macrophage in animal pathological model of spleen deficiency caused by Rhubarb (P<0.01), and it showed a dosage-effect relationship. Conclusion: Cangpo oral liquid has the functions of anti-diarrhea, astringing intestine, anti-inflammatory, anti-nociception, and immunity effect, which provides the pharmacodynamic basis for treating calf spleen deficiency diarrhea in clinic.
