Experimental Research of Sensitization Potential of Five Kinds of Traditional Chinese Medicine Injection with Clearing-heat Function by Mice Popliteal Fossa Lymph Node Assay

Abstract:

Objective: To study sensitization potential of five kinds of traditional Chinese medicine injections (TCMI) with clearing-heat function by using the BALB/c popliteal lymph node assay. Method: Seventy female BALB/c mice were randomly divided into positive group (mercuric chloride), negative control group (normal saline) and the traditional Chinese medicine injection (TCMI) groups, mice were injected subcutaneously (50 μL) with drugs into the right pad of hind foot. Dosages of each animal from normal saline group and mercuric chloride group were 50 μL 0.9%NaCl and 50 μg HgCl₂, respectively. The traditional Chinese medicine (TCM) injection groups, were given 1.92 mL·kg^-1 Zhongjiefeng injection(ZJF), 0.96 mL·kg^-1 Qingkailing injection(QKL), 0.96 mL·kg^-1 Chuanxinlian injection (CXL), 0.96 mL·kg^-1 Chaihu(CH) and 0.48 mL·kg^-1 Banlangen(BLG). The animals were killed on the 6th day, the popliteal lymph nodes(PLN) were isolated and weighed respectively to calculate the PLN weight index(WI).Then the PLN from four of ten mice in each group were fixed with paraformaldehyde solution for histopathologic examination;the other PLN were calculated cell index(CI). Result: Compared to untreated sides, WI and CI of the PLN from mercuric chloride group (WI=3.82±0.93, CI=7.40±0.63), Zhongjiefeng injection(ZJF, WI=2.21±0.15, CI=5.23±0.18), Qingkailing injection(QKL, WI=2.13±0.10, CI=5.17±0.18) and Chuanxinlian injection (CXL, WI=2.06±0.14, CI=5.04±0.27) reached to WI>2 and CI>5(P<0.05 or P<0.01)and Compared to normal saline groups(WI=1.10±0.14, CI=1.23±0.11), their PLN were significantly enlarged, cells were increased.And their pathological examination showed that treated side PLN were enlarged with remarkable germinal center and increased high endothelial venules proliferation.And the responses in Chaihu injection (CH, WI=1.39±0.16, CI=3.15±0.21) or Banlangen injection (BLG, WI=1.38±0.13, CI=3.25±0.36)were unreached to positive response standard, corresponding change was not obvious. Conclusion: ZJF, QKL and CXL have the allergenic potential the sensitization of CH or BLG is not obvious.