Effect of Carbomer on Enzymolysis of Total Flavonoids from Epimedii Folium

Abstract:

Objective: To investigate effect of carbomer on enzymolysis of total flavonoids from Epimedii Folium. Method: Total flavonoids from Epimedii Folium and carbomer was mixed, then hydrolyzed by snailase, HPLC was adopted to observe content change of main flavonoids (including epimedin A, epimedin B, epimedin C, icariin and so on) and main hydrolysates (sagittatoside A, sagittatoside B, 2"-O-rhamnosylicariside Ⅱ, baohuoside Ⅰ and icaritin in enzymolysis liquid, and compared with enzymatic process (no carbomer) of total flavonoids from Epimedii Folium. Result: In a buffer solution (containing 1% carbomer, 37 ℃), hydrolysis rates of epimedin A, epimedin B, epimedin C and icariin were slower, it need about 4 h to completely transformed to secondary glycosides, which was twice in comparison with absense of carbomer;The content of baohuoside Ⅰ was always higher and contents of sagittatoside A and 2"-O-rhamnosylicariside Ⅱ was also higher during 2-6 h than absense of carbomer.The highest content of baohuoside Ⅰ was 48.43 mg·L^-1 and the final stable content was 12.99 mg·L^-1, which were 2.2 and 7.2 times as many as absense of carbomer, respectively.Afterwards, secondary glycosides could continue to be hydrolyzed to aglycone, their production rates were almost the same, which were about 7.60 mg·L^-1·h^-1. Conclusion: Carbomer could slow down enzymolysis rates of total flavonoids from Epimedii Folium, promote production of secondary glycosides, but had no effect on production of aglycone.