Research of Skin Penetration Properties and Toxicology of Natural Hirudin Hydrogels

Abstract:

Objective: To discuss feasibility of permeation percutaneous administration of natural hirudin hydrogels and its toxic action on animal skin. Method: Natural hirudin hydrogels was prepared, improved Franz diffusion cells was adopted to make a permeability test, activity of natural hirudin was determined by Markwardt.Health Wistar rats were adopted to take an acute position skin test and single or multiple dose skin irritation test, health guinea pigs were used to make skin sensitization test. Result: Fibrinogen clotting times of experimental groups were delayed with P<0.05, it reached the maximum of (85.4±1.454) s at 3 h, while the control group had no significant delay;High-dose of natural hirudin hydrogels in short-term had no toxicity for skin, and no irritation for intact skin and broken skin of Wistar rats, no sensitization for intact skin of guines pigs. Conclusion: Natural hirudin could penetrate into intact skin tissue, its gels was safety for skin topical administration of experimental animals.