Optimization of Ultrafine Pulverization Technology of Codonopsis Radix and Investigation of Its Dissolution

Abstract:

Objective: To optimize ultrafine pulverization process of Codonopsis Radix and investigate its in vitro dissolution. Method: Taking extraction amount of lobetyolin as index, orthogonal test was adopted to optimize ultrafine pulverization process with powder consumption, preliminary powder fineness and pulverization time as factors.HPLC was employed to determine the content of lobetyolin with mobile phase of acetonitrile-water (20:80) and detection wavelength of 268 nm.Dissolution was determined by Paddle-shaped mixing method, the content of lobetyolin in stripping liquid at different times from ultrafine powder and ordinary powder was compared. Result: Optimal ultrafine pulverization technology was as follows:preliminary powder fineness 65 mesh, powder consumption 1 000 g, pulverization time 40 minutes;Average extraction amount of lobetyolin was 3.95 mg·g^-1 with RSD of 2.85%.Compared with ordinary powder, the content of lobetyolin in stripping liquid from ultrafine powder increased by 35%, dissolution of ultrafine powder in 30 min was up to 80%, while it was 40% in 120 min for ordinary powder. Conclusion: This optimized technology was stable and feasible, dissolution rate of lobetyolin in ultrafine powder was much larger than ordinary powder.