Optimization of Softening Cutting Process of Daphne giraldii by Orthogonal Design

Abstract:

Objective: To optimize softening cutting process of Daphne giraldii. Method: With extraction amounts of daphnetin, umbelliferone, total coumarins and syringin as indexes, effects of adding water content, softening time and cutting thickness on softening cutting process were investigated by orthogonal design.Contents of daphnetin, umbelliferone and syringin were determined by HPLC, but the content of total coumarins was determined by UV. Result: The best softening cutting process was as follows:adding water amount 0.8 times, softening time 0.5 h and cutting thickness 5 mm;Extraction amounts of daphnetin, umbelliferone, syringin and total coumarins were 1.058, 0.669, 5.793, 3.640 mg·g^-1, respectively. Conclusion: This optimized process was stable and feasible, which could provide process parameters for industrial production and quality control of D. giraldii.