UV on-line Detection of Six Flavonoids Index Ingredients in Zhou’s Prescription

Abstract:

Objective: To establish the rapid UV on-line detection for six flavonoids ingredi-ents of zhou's prescription. Method: Six flavonoids index ingredients contents (catechin, hydroxysafflor yellow A, hyperoside, isoquercitrin, quercitrin quercetin) in 39 batches of zhou's extracting solution were measured by HPLC. The UV spectra of the same samples' were scanned and take the UV absorbance value among 200 nm to 500 nm every 1 nm. The wavelength which were detected by the above method and the six flavonoids index ingre-dients contents which were determined by HPLC were chosen to stepwise regress analyze and establish the prediction equations of six flavonoids index ingredients.Other 8 batches of extracting solution of zhou's prescription were determined by HPLC and UV prediction to test the UV prediction method. Result: The correlation coefficients of equations of eac-h flavonoids index ingredients were:catechin, 0.935;hydroxysafflor yellow A:0.963;hyp-eroside:0.911;isoquercitrin:0.956;quercitrin:0.926;quercetin:0.890.The correlati-on coefficients of six flavonoids contents determined by HPLC and UV prediction were:catechin, 0.946 5;hydroxysafflor yellow A:0.976 5;hyperoside:0.907 9;isoquercitrin:0.981 9;quercitrin:0.911 4;quercetin:0.896 2.The deviation of six flavonoids index ingredi-ents contents measured by the two methods were low.T-test show that there was no significant difference between UV prediction and HPLC method. Conclusion: The result of UV prediction was reliable and accurate, the UV prediction method was rapide and convenient, so it can be used for on-line testing six flavonoids ingredients in producting of Zhou's prescription.