Comparison of High-performance Liquid Chromatography Profiles of Different Formulations of Danggui Buxue

Abstract:

Objective: To compare the formulations of Danggui Buxue decoction, granule, oral liquid of 3 different preparation of various composition differences, which is used for the contradistinction of affects for chemical compositions of different pharmaceutical technologies. Method: 9 samples of formulations of Danggui Buxue were extracted by 5% methanol-water solution. Sample solutions were determined by Lab Alliance high performance liquid chromatograph instrument equipped with Kromasil C₁₈ column and UV detector, and gradient eluted with methanol-0.4% formic acid aqueous solution as mobile phase. The flow rate was set to 1 mL·min^-1, and the wavelength for detection was set to 280 nm. Through the comparison of HPLC characteristic spectrum, and the use of traditional Chinese medicine fingerprint digital information processing technology of Danggui Buxue Decoction, difference analysis on granules, specific, main components and unknown components was performed. Result: It had obvious characteristics for the common peaks of the HPLC chromatographic profiles for the formulations of Danggui Buxue. 15 common peaks were detected. The similarity indexes had some differences between different formulations of Danggui Buxue preparations. The amount and areas of peaks in dispensing granules and oral liquor had different degrees of reduction, the proportion of various components with different. Conclusion: Compared with the decoction, chemical compositions in Danggui Buxue dispensing granules and oral liquor are subject to varying degrees of loss in preparation process, and may affect the clinical efficacy. The HPLC chromatographic profiles method appeared to be simple, accurate and can be used for the quality researches of the formulations of Danggui Buxue.