Evaluation of Chemical Contents, Fingerprint and Antimicrobial Activity of Andrographis paniculata (Burm. f.) Nees Harvested in Different Regions

Abstract:

Objective: The aim of the study was to evaluate the quality of Andrographis paniculata (Burm. f.) Nees harvested in different regions of China. Method: The inhibitory effects of A. paniculata on Shigella dysenteriae growth were investigated by microcalorimetry. UPLC fingerprints of the A. paniculata samples were acquired and then analyzed the correlation with the antimicrobial activity by partial least squares (PLS) analysis. Result: Compared with the control group, all A. paniculata samples from different regions showed inhibitory effects to S. dysenteriae with extending of growth time and decrease of metabolism. And the samples from Yunnan Xishuangbanna had stronger antimicrobial effects than others. According to PLS analysis, there were four chemical contents correlative to the activity of antimicrobial effect. Conclusion: The harvest region had significant influence to the quality of A. paniculata and Yunnan, Hainan, Sichuang and Guangxi were the good harvest regions. The combined evaluation of biological activity and chemical fingerprint provides ability to integrally evaluate the quality of A. paniculata.