Quality Standard of Glycyrrhizic Acid Ammonium Salt in Yanlishuang Kouhan Diwan

Abstract:

Objective: The identification and determination method were established for Yanlishuang Kouhan Diwan. Method: The TLC method was used for identifying Glycyrrhizic acid ammonium salt;The HPLC method was used for determination of Glycyrrhizic acid ammonium salt in yanlishuang Kouhan Diwan, Butyl paraben as internal standard.Diamonsil C₁₈ column was adopted.The mobile phase was Acetonitrile-0.01 mol·L^-1 phosphoric acid (38:62) at a flow rate of 1.0 mL·min^-1.The detection wavelength was 250 nm and column temperture was 25 ℃. Result: This method is specfic and accurate to Glycyrrhizic acid ammonium salt in Yanlishang Kouhan Diwan.The linear range of Glycyrrhizic acid ammonium salt within 0.212-3.71 mg (r=0.999 9), The average recovery was 101.07%. Conclusion: The method is convenient, sensitive and accurate, which could be used for quality control of the drug.