Separation and Determination of Betulinic Acid in Adenosma indianum (Lour.) Merr. by HPLC

Abstract:

Objective: To study the chemical constituents of Adenosma indianum (Lour.) Merr. and the contents of betulinic acid in Adenosma indianum (Lour.) Merr. by HPLC. Method: The chemical constituents were isolated by column chromatography over silica ge1.The Structures of the compound was elucidated by spectroscopic and chromatographic analysis.SHISEIDO-SPOLAR C₁₈ column (4.6 mm×250 mm, 5 μm) was used with methanol-0.2% phosphoric acid (82:18) as mobile phase. The flow rate was 1.0 mL·min^-1.The UV detection wavelength was 205 nm and the column temperature was 35 ℃. Result: The compound was identified as betulinic acid.The linear response range of betulinic acid were 0.048-0.48 μg, respectively.The average recovery of this compound was 99.84%, RSD 1.35%. Conclusion: The betulinic acid was isolated from Adenosma indianum (Lour.) Merr. for the first time.This established method in this study is simple, reliable, reproducible and accurate for the analysis of betulinic acid in Adenosma indianum (Lour.) Merr.