Quantitative Determination and Orthogonal Test of Water Extraction Conditions for Epicatechin in Leaves of Breynia fruticosa

Abstract:

Objective: To establish the determination method and optimize the water extraction conditions for Epicatechin in leaves of Breynia fruticosa. Method: The content of Epicatechin in leaves of Breynia fruticosa was determined by using HPLC. Permeating time, ratio of solid to liquid, extraction time and extraction times for the affection of water extraction rate of Epicatechin in leaves of Breynia fruticosa were detected by the orthogonal test. Result: The linear range of Epicatechin was 0.006 6-0.264 0 g·L^-1, the standard curve of Epicatechin was as follows Y=59 283X+53.986, r=0.999 9, the average recovery of Epicatechin was 98.2% with RSD 1.03%. The optimal water extraction technics were as follows:ratio of solid to liquid at 1:20 (w/v) and permeating for 45 minutes, 80 ℃ extraction 2 times and 1 hour for each time. Conclusion: HPLC method for determination of Epicatechin in leaves of Breynia fruticosa is simple and selective.The content of Epicatechin was higher after orthogonal test, which can be used for the development of Breynia fruticosa.